Fig. 4

a The thermal stability of the linear and cyclized lichenase at various temperature intervals of 50, 55 and 60 °C. b Enzyme activity after incubation at room temperature for 10 min or 100 °C heating. Enzyme activity was indicated by the generation rate of product. cEB cyclized lichenase, lEB linear lichenase, TlEB linear topology of product after TEV cleavage following cyclization. Experiments were performed in triplicate and error bar means the standard deviation