Engineering of a high lipid producing Yarrowia lipolytica strain

Table 3 Lipid production parameters calculated for the final time point (120 h) of the batch and fed-batch fermentation experiments with strains NS18 and NS432

	NS18		NS432
	Batch	Fed-batch	Batch	Fed-batch
Hours post-inoculation	116	116	114	115
Lipid content (% w lipid/w DCW)	24 %	25 % ± 4 %	77 % ± 2 %	73 % ± 2 %
Titer (g lipid/L)	3.6	12.8 ± 1.0	30.8 ± 1.8	84.5 ± 1.7
Productivity (g lipid L⁻¹ h⁻¹)	0.03	0.11 ± 0.01	0.27 ± 0.02	0.73 ± 0.01
Cell specific productivity^a (g lipid g lipid free DCW⁻¹ h⁻¹)	0.002	0.003 ± 0.001	0.048 ± 0.006	0.044 ± 0.001
Yield (g lipid/g glucose consumed)^b	0.06	0.07 ± 0.01	0.21 ± 0.004	0.20 ± 0.004

All experiments except for NS18—batch were performed twice and the average with standard deviation from duplicate experiments is shown
^a Cell specific productivity calculated over 20–70-h post-inoculation
^b Yields calculated for batch cultivation account for a measured 10 % volume loss due to sampling and aeration over the course of fermentation

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