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Table 3 Lipid production parameters calculated for the final time point (120 h) of the batch and fed-batch fermentation experiments with strains NS18 and NS432

From: Engineering of a high lipid producing Yarrowia lipolytica strain

  NS18 NS432
Batch Fed-batch Batch Fed-batch
Hours post-inoculation 116 116 114 115
Lipid content (% w lipid/w DCW) 24 % 25 % ± 4 % 77 % ± 2 % 73 % ± 2 %
Titer (g lipid/L) 3.6 12.8 ± 1.0 30.8 ± 1.8 84.5 ± 1.7
Productivity (g lipid L−1 h−1) 0.03 0.11 ± 0.01 0.27 ± 0.02 0.73 ± 0.01
Cell specific productivitya (g lipid g lipid free DCW−1 h−1) 0.002 0.003 ± 0.001 0.048 ± 0.006 0.044 ± 0.001
Yield (g lipid/g glucose consumed)b 0.06 0.07 ± 0.01 0.21 ± 0.004 0.20 ± 0.004
  1. All experiments except for NS18—batch were performed twice and the average with standard deviation from duplicate experiments is shown
  2. a Cell specific productivity calculated over 20–70-h post-inoculation
  3. b Yields calculated for batch cultivation account for a measured 10 % volume loss due to sampling and aeration over the course of fermentation
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