Fig. 5

Targeted metabolomic analysis of wild-type and BHBT5 strains. a Methylotrophic major metabolism in M. extorquens AM1 and metabolite change between BHBT5 and wild-type. The colored circle indicates significant difference (p < 0.05). The metabolites without significant change are shown in green. Double arrows indicate multiple reactions. b The ratio of metabolite abundance of the BHBT5 strain to the wild-type strain. Note: myristic acid (C14:0) was not presented as it was only detected in the wild-type strain. Ratios outside the 1.0 ± 0.2 range indicated significant difference of concentration (p < 0.05) for each metabolite (purple). The data were presented as the mean plus STDEV calculated from triplicate biological replicates