Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance

Table 4 Differential metabolites were discovered by PLS-DA

GC-MS compound	Match value/elemental composition	m/z	Retention time (min)	Peak abundance
GC-MS compound	Match value/elemental composition	m/z	Retention time (min)	Wild-type BHBT5
Cellobiose	884	361, 217, 204	38.46	NA	1.85E+06
Trehalose	830	331, 191	37.11	NA	3.61E+05
Mannose	910	387, 357	32.61	NA	1.70E+06
LC–MS compound
Unknown	C₂₀H₃₃NO₃	336.2534	2.66	NA	1.38E+06
Unknown	C₁₆H₃₀N₂O₃	299.2335	2.82	NA	8.83E+04
Unknown	C₁₄H₂₇N₃	238.2277	14.00	NA	1.40E+05
Unknown	C₁₀H₁₉NO₃	202.1438	2.49	NA	2.27E+05
Unknown	C₁₀H₁₉NO₃	200.1296	2.53	NA	6.20E+05
Analog of farnesol	C₁₂H₂₀O₂	197.1535, 179.1447	3.42	2.57E + 05	NA

The wild-type and BHBT5 strain were grown on methanol with the addition of 0 and 0.5 % 1-butanol, respectively
NA not available due to the abundance lower than the limit of detection

ISSN: 2731-3654