Expression and characterization of a novel 1,3-regioselective cold-adapted lipase from Rhizomucor endophyticus suitable for biodiesel synthesis

Table 2 Substrate specificity of the purified lipase (ReLipA) from R. endophyticus

Substrate	Chain length	Specific activity (U mg⁻¹)	Relative activity (%)
pNP esters
pNPA	C₂	0	0
pNPB	C₄	852	53
pNPH	C₆	941	58
pNPC	C₈	1611	100
pNPD	C₁₀	1137	71
pNPL	C₁₂	1297	81
pNPM	C₁₄	936	58
pNPP	C₁₆	277	17
Triglycerides
Triacetin	C₂	0	0
Tributyrin	C₄	1164	64
Tricaproin	C₆	1167	64
Tricaprylin	C₈	1823	100
Tricaprin	C₁₀	715	39
Trilaurin	C₁₂	421	23
Trimyristin	C₁₄	106	6
Tripalmitin	C₁₆	44	2.4

Enzyme’s activity was determined in 50 mM citrate buffer (pH 6.0) at 40 °C for 10 min

ISSN: 2731-3654