Fig. 4
From: Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production
Optimization of oxygen supply in 5-L fermentor. BSF9 was cultured in LBR medium with glucose at 37 °C and 300 rpm in a 5-L fermenter at different aeration rates. Filled square glucose concentration; Filled circle 2,3-BD concentration; Filled triangle AC concentration; Filled diamond cell density, optical density at 600 nm (OD600). Cultivation was carried out at an initial pH of 6.5. Agitation speed was 300 rpm. Batch fermentation with aeration rates of a 1 vvm; b 0.5 vvm; c 0.1 vvm; d glucose consumption, meso-2,3-BD production, AC, meso-2,3-BD yield, and AC + BD total yield of BSF9 during oxygen-supply optimization in batch fermentation process