Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production

Fu, Jing; Huo, Guangxin; Feng, Lili; Mao, Yufeng; Wang, Zhiwen; Ma, Hongwu; Chen, Tao; Zhao, Xueming

doi:10.1186/s13068-016-0502-5

Biotechnology for Biofuels and Bioproducts

Table 1 Metabolic characterizations of B. subtilis strains cultivated in M9 medium supplemented with 10 g/L glucose under the microaerobic condition

From: Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production

Strain	CDW^a,c (g/L)	2,3-BD^b,c (g/L)	AC^b,c (g/L)	Acetate^b,c (g/L)	Lactate^b,c (g/L)	Succinate^b (g/L)	Glucose uptake rate^c [g/(L h)]	Time for glucose depletion^c (<0.2 g/L) (h)	2,3-BD productivity^c (g/(L h))	2,3-BD yield^c (mol/mol)	AC + 2,3-BD (at 60 h) (g/L)	AC + 2,3-BD (at 96 h) (g/L)
BSF1^c	0.960 ± 0.012	2.70(D) ± 0.05	0.82 ± 0.06	0.24 ± 0.03	0.40 ± 0.04	0.08 ± 0.00	0.270	37	0.073	0.54	4.10 + 0.27	2.90 + 0
BSF2	0.920 ± 0.005	2.84(D) ± 0.11	1.03 ± 0.07	0.20 ± 0.02	0.36 ± 0.01	0.09 ± 0.01	0.260	38.5	0.074	0.57	4.20 + 0.19	3.95 + 0
BSF3^c	0.898 ± 0.015	ND^d	3.83 ± 0.02	0.17 ± 0.01	0.44 ± 0.03	0.07 ± 0.00	0.233	43	0	0	4.21 + 0	4.00 + 0
BSF4	0.828 ± 0.011	ND	3.70 ± 0.05	0.14 ± 0.01	0.34 ± 0.05	0.11 ± 0.01	0.222	45	0	0	3.87 + 0	3.64 + 0
BSF5	0.814 ± 0.009	ND	3.71 ± 0.10	0.71 ± 0.01	ND	0.29 ± 0.01	0.208	48	0	0	3.69 + 0	3.50 + 0
BSF6	0.806 ± 0.004	ND	3.88 ± 0.02	0.61 ± 0.01	ND	0.20 ± 0.03	0.200	50	0	0	3.71 + 0	3.52 + 0
BSF7	0.813 ± 0.007	2.85(M) ± 0.12	0.91 ± 0.11	0.73 ± 0.03	ND	0.28 ± 0.02	0.244	41	0.070	0.57	2.14 + 1.83	2.04 + 0.97
BSF8	0.838 ± 0.010	2.90(M) ± 0.05	1.10 ± 0.02	0.65 ± 0.01	ND	0.25 ± 0.02	0.244	41	0.071	0.58	1.93 + 1.58	1.90 + 0.88
BSF9	0.846 ± 0.008	3.56(M) ± 0.08	0.68 ± 0.05	0.56 ± 0.04	ND	0.19 ± 0.01	0.256	39	0.091	0.71	1.70 + 2.43	1.72 + 1.93
BSF17	0.970 ± 0.008	2.93(M) ± 0.09	1.04 ± 0.08	0.18 ± 0.02	0.66 ± 0.03	0.05 ± 0.00	0.270	37	0.079	0.59	2.42 + 1.56	2.51 + 0.81
BSF18^c	0.945 ± 0.009	3.01(D) ± 0.06	0.92 ± 0.09	0.29 ± 0.01	0.69 ± 0.03	0.06 ± 0.00	0.260	38	0.079	0.60	3.92 + 0.11	3.85 + 0
BSF25	0.969 ± 0.012	3.28(M) ± 0.04	0.85 ± 0.07	0.14 ± 0.01	0.25 ± 0.02	0.06 ± 0.00	0.278	36	0.091	0.66	2.96 + 1.31	2.53 + 0.94

Bacteria were cultivated with 100 mL M9 in a 250-mL flask at an agitation speed of 100 rpm and 37 °C; Data are average values and standard deviations of triplicate experiments. CDW cell dry weight, 2,3-BD 2,3-butanediol, D d-(−)-2,3-BD, M meso-2,3-BD; ND not detected or lower than 0.01 g/L
^aThe CDW was calculated using the relationship “CDW = OD₆₀₀*0.325”
^bThese metabolites were measured when the residual glucose almost reached zero (<0.2 g/L)
^cThese results for BSF1, BSF3, and BSF18 were published in a previous paper (Fu et al. [25]), and are listed here for relative discussion in this paper

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