Table 3 Strains and plasmids

Strains

 B. subtilis 168

Wide-type strain, trpC2

BGSC^a

 E. coli DH5α

Invitrogen

K. pneumoniae CICC 10011

CICC^b

 BSF1

B. subtilis 168Δupp

(Fu et al. [25])

 BSF2

B. subtilis 168ΔuppΔacoA

This study

 BSF3

B. subtilis 168ΔuppΔacoAΔbdhA

(Fu et al. [25])

 BSF4

B. subtilis 168ΔuppΔacoAΔbdhAΔpta

This study

 BSF5

B. subtilis 168ΔuppΔacoAΔbdhAΔptaΔldh

This study

 BSF6

B. subtilis 168ΔuppΔacoAΔbdhAΔptaΔldh; P ₄₃ ::alsS-alsD

This study

 BSF7

B. subtilis 168ΔuppΔacoAΔbdhAΔptaΔldh; P ₄₃ ::budC, spc

This study

 BSF8

B. subtilis 168ΔuppΔacoAΔbdhAΔptaΔldh; P ₄₃ ::alsS-alsD; P ₄₃ ::budC, spc

This study

 BSF9

B. subtilis 168ΔuppΔacoAΔbdhAΔptaΔldh; P ₄₃ ::alsS-alsD; P ₄₃ ::alsS-alsD-budC-udhA, erm

This study

 BSF17

B. subtilis 168ΔuppΔacoAΔbdhA; pHP13-P ₄₃ -budC

This study

 BSF18

B. subtilis 168ΔuppΔacoAΔbdhA; pHP13-P ₄₃ -bdhA

(Fu et al. [25])

 BSF25

B. subtilis 168ΔuppΔacoAΔbdhA; P ₄₃ ::alsS-alsD-budC-udhA, erm

This study

Plasmids

 pUC18

Amp^r

Lab Stock

 pUC18-uppFB-neo

Kan^r

Lab Stock

 pC194

Cm^r, Bacillus cloning vector

Lab Stock

 pE194

Em^r, Bacillus cloning vector

Lab Stock

 pHP13

Cm^r, Em^r, E. coli–B. subtilis shuttle vector

Lab Stock

 pUC18-SP

Amp^r, Spc^r, containing P ₄₃ promoter

Lab stock

 pUC18-SP-budC

Amp^r, Spc^r, containing P ₄₃ promoter and budC

This study

 pCU

puC18 containing cat-upp cassette

Lab stock

 pCU-ptaFB

pCU containing pta flanks

This study

 pCU-ldhFB

pCU containing ldh flanks

This study

 pCU-bdhAFB-PSD

pCU-bdhAFB containing alsS-alsD under P ₄₃

This study

 pUC18-SP-budC

Amp^r, Spc^r, containing budC under P ₄₃ promoter

This study

 pUC18-P ₄₃

Amp^r, Em^r, containing P ₄₃ promoter

This study

 pUC18-P ₄₃ -SD

Amp^r, Em^r, containing alsS and alsD under P ₄₃ promoter

This study

 pUC18-P ₄₃ -SDU

Amp^r, Em^r, containing alsS, alsD and udhA under P ₄₃ promoter

This study

 pUC18-P ₄₃ -SDBU

Amp^r, Em^r, containing alsS, alsD, budC, and udhA under P ₄₃ promoter

This study

 pPSDBUE

pUC18-P ₄₃ -SDBU containing erythromycin resistance gene

This study

 pHP13-P ₄₃ -budC

Em^r, Cm^r, containing budC under P ₄₃ promoter

This study