Fig. 1

Isoprene production rate and ispS expression in standard medium (0 % NaCl) of the different Synechocystis strains. a Isoprene production is expressed in relation to optical density (OD₇₅₀; a measure of cell number) over 24 h of phototrophic growth in the Synechocystis strains carrying various constructs for isoprene synthesis (see Table 1). Mean values and standard deviation from three independent growth experiments with each two technical replicates are given. Statistical significant differences (p ≤ 0.05) between strain # 642 and other are marked by asterisk. b Expression of the ispS gene in the different Synechocystis strains. The relative expression (rnpB amount was used as internal loading control) of ispS was estimated by qPCR. The expression in strain # 643 was set to 1. c Accumulation of the IspS protein in the different Synechocystis strains. The protein amounts were visualized by immune-blotting. Equal amounts of soluble protein (10 μg) were loaded on gels. The blot was incubated with a specific IspS antibody and the IspS protein was visualized by chemoluminescence. d Coomassie-stained SDS-PAGE of proteins which correspond to the Western Blot above