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Table 1 Transcriptional changes of genes coding for the six key enzymes for alcohol production

From: Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach

Metabolic state/gene Control ΔadhE1 ΔadhE2
Acidogenesis
 adhE1 (CA_P0162) 0.09 ± 0.01 0 ± 0 0.2 ± 0.01
 adhE2 (CA_P0035) 0.42 ± 0.02 2.31 ± 0.6 0 ± 0
 bdhA (CA_C3299) 8.15 ± 0.32 4.33 ± 1.03 5.76 ± 0.2
 bdhB (CA_C3298) 16.31 ± 0.45 5.13 ± 4.28 1.52 ± 0.11
 bdhC (CA_C3392) 8.63 ± 0.94 7.55 ± 0.28 17.65 ± 0.44
 pdc (CA_P0025) 5.6 ± 0.81 1.74 ± 0.1 3.23 ± 0.24
Solventogenesis
 adhE1 (CA_P0162) 7.09 ± 0.73 0 ± 0 11.4 ± 4.71
 adhE2 (CA_P0035) 0.21 ± 0.02 26.6 ± 0.26 0 ± 0
 bdhA (CA_C3299) 8.22 ± 1.33 4.62 ± 0.06 7.55 ± 0.75
 bdhB (CA_C3298) 28.1 ± 5.07 34.78 ± 1.55 17.76 ± 2.83
 bdhC (CA_C3392) 11.28 ± 1.68 12.52 ± 0.36 9.16 ± 0.67
 pdc (CA_P0025) 5.17 ± 2.78 6.59 ± 0.3 6.23 ± 1.03
Alcohologenesis
 adhE1 (CA_P0162) 0.13 ± 0.01 0 ± 0 0.18 ± 0.01
 adhE2 (CA_P0035) 68.6 ± 12.95 62.56 ± 7.58 0 ± 0
 bdhA (CA_C3299) 6.08 ± 0.37 4.82 ± 0.13 7.39 ± 0.21
 bdhB (CA_C3298) 14.33 ± 2.65 16.96 ± 0.25 15.16 ± 0.46
 bdhC (CA_C3392) 10.73 ± 0.94 11.05 ± 0.25 8.95 ± 0.32
 pdc (CA_P0025) 1.23 ± 0.51 0.83 ± 0.03 1.86 ± 0.07
  1. The numbers of mRNA molecules per cell are shown as mean values ± SD from three biological replicates