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Table 1 Transcriptional changes of genes coding for the six key enzymes for alcohol production

From: Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach

Metabolic state/gene

Control

ΔadhE1

ΔadhE2

Acidogenesis

 adhE1 (CA_P0162)

0.09 ± 0.01

0 ± 0

0.2 ± 0.01

 adhE2 (CA_P0035)

0.42 ± 0.02

2.31 ± 0.6

0 ± 0

 bdhA (CA_C3299)

8.15 ± 0.32

4.33 ± 1.03

5.76 ± 0.2

 bdhB (CA_C3298)

16.31 ± 0.45

5.13 ± 4.28

1.52 ± 0.11

 bdhC (CA_C3392)

8.63 ± 0.94

7.55 ± 0.28

17.65 ± 0.44

 pdc (CA_P0025)

5.6 ± 0.81

1.74 ± 0.1

3.23 ± 0.24

Solventogenesis

 adhE1 (CA_P0162)

7.09 ± 0.73

0 ± 0

11.4 ± 4.71

 adhE2 (CA_P0035)

0.21 ± 0.02

26.6 ± 0.26

0 ± 0

 bdhA (CA_C3299)

8.22 ± 1.33

4.62 ± 0.06

7.55 ± 0.75

 bdhB (CA_C3298)

28.1 ± 5.07

34.78 ± 1.55

17.76 ± 2.83

 bdhC (CA_C3392)

11.28 ± 1.68

12.52 ± 0.36

9.16 ± 0.67

 pdc (CA_P0025)

5.17 ± 2.78

6.59 ± 0.3

6.23 ± 1.03

Alcohologenesis

 adhE1 (CA_P0162)

0.13 ± 0.01

0 ± 0

0.18 ± 0.01

 adhE2 (CA_P0035)

68.6 ± 12.95

62.56 ± 7.58

0 ± 0

 bdhA (CA_C3299)

6.08 ± 0.37

4.82 ± 0.13

7.39 ± 0.21

 bdhB (CA_C3298)

14.33 ± 2.65

16.96 ± 0.25

15.16 ± 0.46

 bdhC (CA_C3392)

10.73 ± 0.94

11.05 ± 0.25

8.95 ± 0.32

 pdc (CA_P0025)

1.23 ± 0.51

0.83 ± 0.03

1.86 ± 0.07

  1. The numbers of mRNA molecules per cell are shown as mean values ± SD from three biological replicates