Fig. 3

HPAEC–PAD analysis of inulin hydrolysates catalyzed by extracellular inulinase of engineered strains. The strains were cultured in YPD medium. Culture supernatant at 48 h was incubated with 2 % of inulin (w/v) for 8 h at 55 °C. The reaction mixture was analyzed by HPAEC–PAD. Inulin solution was used as the control. The arrows denoted the oligosaccharides hydrolyzed by exo-inulinase or generated from long-chain inulin molecules by endo-inulinase digestion