Fig. 6

Dependency of Tafar1-5copy-Δdga1 fao1 strain’s growth and intracellular hexadecanol production on carbon source and C/N ratio. Hexadecanol-producing capability (a), cell growth (b), and hexadecanol production (c) of the engineered Tafar1-5copy-Δdga1 fao1 strain in basal SD medium with altered combination of carbon and nitrogen source were detected over 5 days. Pre-culture of the strain (SD medium) was inoculated into fourteen medium formulations containing glucose/glycerol (between 20 and 160 g/L) and ammonium (between 0.055 and 1.365 g/L) in the initial OD₆₀₀ of 0.05 for cultivations and detections. These studies were conducted twice and results of one batch were presented