Table 1 List of plasmids and strains used in this study
From: A water-forming NADH oxidase regulates metabolism in anaerobic fermentation
Plasmid/strain | Genotype | Source |
|---|---|---|
Plasmid | ||
pYX212 | 2 μ, TPI promoter, AMPR | A gift from Pro. Yingjin Yuan (Tianjin University, Tianjin, China) |
pYX212-NOX | pYX212 with noxE from L. lactis | This study |
pSY8 | Stored in our laboratory | |
pSY8-NOX | pSY8 with noxE from L. lactis | This study |
Strain | ||
S. cerevisiae BY4741 | MATa;ura3;his3;leu2;met15 | A gift from Pro. Yingjin Yuan (Tianjin University, Tianjin, China) |
Sce-CON | BY4741/pYX212 | This study |
Sce-NOX | BY4741/pYX212-NOX | This study |
C. acetobutylicum 428 | Stored in our laboratory | |
Cac-CON | C. acetobutylicum 428/pSY8 | This study |
Cac-NOX | C. acetobutylicum 428/pSY8-NOX | This study |