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Table 1 Purification steps using immobilized metal affinity chromatography (IMAC) of recombinant LPMOs

From: Salt-responsive lytic polysaccharide monooxygenases from the mangrove fungus Pestalotiopsis sp. NCi6

  Purification Volume (mL) Total activity (U) Protein (mg) Specific activity (U g−1) Activity yield Purification factor (fold)
PsLPMOA Culture medium 1500 1.2 495 2.42 100 1.0
IMAC 2.7 0.51 130 3.92 42.5 1.6
PsLPMOB Culture medium 1060 0.25 1160 0.22 100 1.0
IMAC 1.9 0.62 285 2.18 248.0 9.9
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