Table 1 Purification steps using immobilized metal affinity chromatography (IMAC) of recombinant LPMOs
From: Salt-responsive lytic polysaccharide monooxygenases from the mangrove fungus Pestalotiopsis sp. NCi6
Purification | Volume (mL) | Total activity (U) | Protein (mg) | Specific activity (U g−1) | Activity yield | Purification factor (fold) | |
|---|---|---|---|---|---|---|---|
PsLPMOA | Culture medium | 1500 | 1.2 | 495 | 2.42 | 100 | 1.0 |
IMAC | 2.7 | 0.51 | 130 | 3.92 | 42.5 | 1.6 | |
PsLPMOB | Culture medium | 1060 | 0.25 | 1160 | 0.22 | 100 | 1.0 |
IMAC | 1.9 | 0.62 | 285 | 2.18 | 248.0 | 9.9 |