Engineering Bacillus licheniformis for the production of meso-2,3-butanediol

Table 1 Production of various metabolites by different B. licheniformis strains

B. licheniformis strain	Biomass (OD₆₀₀)	Metabolites (g/L)						meso-2,3-BD yield^a (%)	meso-2,3-BD productivity^b (g/L–h)
B. licheniformis strain	Biomass (OD₆₀₀)	meso-2,3-BD	D-2,3-BD	Acetoin	Lactic acid	Acetic acid	Ethanol	meso-2,3-BD yield^a (%)	meso-2,3-BD productivity^b (g/L–h)
WX-02	29.60 ± 0.82	18.77 ± 0.33	12.51 ± 0.36	10.33 ± 0.02	2.21 ± 0.12	0.46 ± 0.06	0.73 ± 0.12	15.64	0.78
WX-02Δgdh	28.80 ± 0.24	25.86 ± 3.00	ND	17.19 ± 0.37	2.13 ± 0.02	0.21 ± 0.01	0.54 ± 0.03	21.55	1.08
WX-02ΔacoR	28.05 ± 0.88	19.54 ± 0.69	13.10 ± 1.19	11.96 ± 0.91	1.94 ± 0.07	0.25 ± 0.07	0.62 ± 0.08	16.29	0.81
WX-02ΔgdhΔacoR	30.03 ± 0.69	28.22 ± 0.82	ND	17.16 ± 0.66	1.63 ± 0.17	0.11 ± 0.04	0.51 ± 0.14	23.52	1.18

Strains were grown in 250-mL flasks containing 50-mL medium and incubated in a rotary shaker with 180 rpm at 37 °C for 24 h. The initial glucose concentration was 120 g/L, which was completely consumed by the cells at the end of culture; Data are presented as mean ± SDs of three replicates
^aThe yield was calculated as the weight ratio of meso-2,3-BD produced to glucose consumed
^bThe productivity was calculated by dividing maximum meso-2,3-BD concentration to the time when the cells were harvested

ISSN: 2731-3654