Table 3 Strains and plasmids used in this study
From: Engineering Bacillus licheniformis for the production of meso-2,3-butanediol
Strain or plasmid | Relevant genotype/description | Source |
|---|---|---|
Strains | ||
 B. licheniformis | ||
  WX-02 | CCTCC M208065, wild-type strain | Laboratory stock |
  WX-02Δgdh | gdh-knock-out mutant of WX-02 | This work |
  WX-02Δgdh/pHY-gdh | recombinant strain of WX-02Δgdh, harboring pHY-gdh, Tcr | This work |
  WX-02ΔacoR | acoR knock-out mutant of WX-02 | This work |
  WX-02ΔgdhΔacoR | gdh and acoR double-deletion mutant of WX-02 | This work |
 E. coli | ||
  DH5α | F– Φ80d/lacZΔM15, Δ (lacZYA-argF) U169, recA1, endA1, hsdR17 (r –K , m +K ), phoA, supE44, λ-, thi-1, gyrA96, relA1 | Laboratory stock |
  BL21(DE3) | F–, ompT, hsdS B (r − B m − B ), gal, dcm (DE3) | Laboratory stock |
  BL21(DE3)/pET-gdh | E. coli BL21(DE3) harboring pET-gdh, Kanr | This work |
Plasmids | ||
 pET-28a(+) | E. coli expressing vector, Kanr | Laboratory stock |
 pET-gdh | pET-28a(+) carrying gdh gene | This work |
 pHY300PLK | E. coli-Bacillus shuttle vector, Ampr in E. coli, Tcr in both E. coli and B. subtilis | Laboratory stock |
 pHY-gdh | pHY300PLK carrying gdh gene | This work |
 T2(2)-ori | E. coli-B. licheniformis shuttle vector, OripUC/Orits, Kanr | Laboratory stock |
 T2Δgdh | T2(2)-ori derivative containing homologous arms for gdh-knock-out | This work |
 T2ΔacoR | T2(2)-ori derivative containing homologous arms for acoR knock-out | This work |