Table 4 Primers used in this study
From: Engineering Bacillus licheniformis for the production of meso-2,3-butanediol
Primer name | Sequence 5′ → 3′a |
|---|---|
Primers for gdh cloning | |
 gdh-F | CATGCCATGGGCATGTCAAAATCAGTAAAATC |
 gdh-R | CCGCTCGAGATCGTGATAAGATTCTGC |
Primers for gdh deletion | |
 Δgdh-A-F | GCTCTAGAGATCTGACGACGAAAACAAG |
 Δgdh-A-R | ACGGAAACGGCTTTTCGTCTAGGTAATTCCCCCTTCACTATC |
 Δgdh-B-F | GATAGTGAAGGGGGAATTACCTAGACGAAAAGCCGTTTCCGT |
 Δgdh-B-R | GCGAGCTCATTATTATGGAGTACCGTGGATC |
 Δgdh-F | TCTATCTGACCTTTGATAACGGCT |
 Δgdh-R | GAATTTAGTGGTTCTGATCTGCATG |
Primers for acoR deletion | |
 ΔacoR-A-F | GGGGTACCTGCCTCAGGCAGAGACATG |
 ΔacoR-A-R | CGTCATGCTTTGGGCGCCGAGAGGTCCATCCTCTCCTCATGCTTTTTC |
 ΔacoR-B-F | GAAAAAGCATGAGGAGAGGATGGACCTCTCGGCGCCCAAAGCATGACG |
 ΔacoR-B-R | CCGCTCGAGGGGTAAATGTGCTTCCTCCGCCT |
 ΔacoR-F | CGGAGTCCAATATGACAGG |
 ΔacoR-R | TCTGATATGAGCCATGACG |
Primers for gdh complementation | |
 P43-gdh-TamyL-1 | GCTCTAGAGCGGAATTTCCAATTTCATG |
 P43-gdh-TamyL-2 | GATTTTACTGATTTTGACATGTGTACATTCCTCTCTTACC |
 P43-gdh-TamyL-3 | GGTAAGAGAGGAATGTACACATGTCAAAATCAGTAAAATC |
 P43-gdh-TamyL-4 | CGTCCTCTCTGCTCTTCTATCTTTTAATCGTGATAAGATTCTG |
 P43-gdh-TamyL-5 | CAGAATCTTATCACGATTAAAAGATAGAAGAGCAGAGAGGACG |
 P43-gdh-TamyL-6 | CGCGGATCCGATCACCCGCGATACCGTC |