Fig. 6

Analysis of xylan utilisation by recombinant C. acetobutylicum strains producing anchored and unanchored minicellulosome constructs. Strains were streaked onto plates containing 30 ml CBM-agar with 1 % of either xylose or xylan as the sole carbon source. Plates were incubated in an anaerobic cabinet at 37 °C for the indicated time and were subsequently removed for imaging. 1 CEL07, overexpressing the native srtA gene; 2 CEL16, overexpressing the native srtA gene and producing the enzymes Xyn10A-FLAG, Cel9G-FLAG, and Cel48F-FLAG, without scaffoldin; 3 CEL19, overexpressing the native sortase gene, producing all three enzymes, and producing the unanchored scaffoldin CipC2F3; 4 CEL21, overexpressing the native sortase gene, producing all three enzymes, and producing the sortase signal-bearing scaffoldin CipC2F3-CA_C0205ss