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Table 1 Kinetic parameters of the chimeric enzyme compared with the parental enzyme

From: A xylose-stimulated xylanase–xylose binding protein chimera created by random nonhomologous recombination

 

Parental XynA

Chimera

−xylose

+xylose

−xylose

+xylose

K aRBB-Xylan

1.7 ± 0.2

1.4 ± 0.1

0.33 ± 0.2

0.33 ± 0.1

k cat/K bRBB-Xylan

0.47 ± 0.03

0.39 ± 0.02

0.86 ± 0.08

1.29 ± 0.03

n H

1.0

1.3

2.4

2.2

  1. Data represent the mean ± SD
  2. amg mL−1
  3. bmL mg−1 s−1