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Table 1 Kinetic parameters of the chimeric enzyme compared with the parental enzyme

From: A xylose-stimulated xylanase–xylose binding protein chimera created by random nonhomologous recombination

  Parental XynA Chimera
−xylose +xylose −xylose +xylose
K aRBB-Xylan 1.7 ± 0.2 1.4 ± 0.1 0.33 ± 0.2 0.33 ± 0.1
k cat/K bRBB-Xylan 0.47 ± 0.03 0.39 ± 0.02 0.86 ± 0.08 1.29 ± 0.03
n H 1.0 1.3 2.4 2.2
  1. Data represent the mean ± SD
  2. amg mL−1
  3. bmL mg−1 s−1