Deep metagenome and metatranscriptome analyses of microbial communities affiliated with an industrial biogas fermenter, a cow rumen, and elephant feces reveal major differences in carbohydrate hydrolysis strategies

Table 1 Number of generated reads and statistics of different de novo assemblies

Biogas fermenter May 2013 sample	#Reads used for assembly	Ray assembly					IDBA-UD assembly
Biogas fermenter May 2013 sample	#Reads used for assembly	#Contigs total	#Contigs >1000 bp	N50 for contigs >1000 bp	Mb in contigs >1000 bp	Mb total	#Contigs total	#Contigs >1000 bp	N50 for contigs >1000 bp	Mb in contigs >1000 bp	Mb total
DNA isolation 1	159,458,382	1,201,371	57,009	7183	209.7	486.0	556,160	116,123	5587	404.7	613.8
DNA isolation 2	421,986,642	2,003,618	94,702	11,536	425.9	876.6	947,772	200,495	5976	724.0	1069.9
DNA isolation 1 + 2	581,445,024	2,319,807	112,571	10,871	512	1,035.8	1,142,608	236,489	5823	843.0	1255.4^a
DNA isolation 2 + additional sequencing of isolation 2	737,631,618	2,593,366	123,435	12,418	581.3	1161.7^b
DNA isolation 1 + 2 + additional sequencing of isolation 2	897,090,000	2,826,937	140,535	11,784	653.73	1292.2

^a Assembly uploaded to IMG/MER and used for the phylogenetic and comparative analyses
^b Assembly used for metagenomic binning

ISSN: 2731-3654