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Table 1 Number of generated reads and statistics of different de novo assemblies

From: Deep metagenome and metatranscriptome analyses of microbial communities affiliated with an industrial biogas fermenter, a cow rumen, and elephant feces reveal major differences in carbohydrate hydrolysis strategies

Biogas fermenter May 2013 sample #Reads used for assembly Ray assembly IDBA-UD assembly
#Contigs total #Contigs >1000 bp N50 for contigs >1000 bp Mb in contigs >1000 bp Mb total #Contigs total #Contigs >1000 bp N50 for contigs >1000 bp Mb in contigs >1000 bp Mb total
DNA isolation 1 159,458,382 1,201,371 57,009 7183 209.7 486.0 556,160 116,123 5587 404.7 613.8
DNA isolation 2 421,986,642 2,003,618 94,702 11,536 425.9 876.6 947,772 200,495 5976 724.0 1069.9
DNA isolation 1 + 2 581,445,024 2,319,807 112,571 10,871 512 1,035.8 1,142,608 236,489 5823 843.0 1255.4a
DNA isolation 2 + additional sequencing of isolation 2 737,631,618 2,593,366 123,435 12,418 581.3 1161.7b  
DNA isolation 1 + 2 + additional sequencing of isolation 2 897,090,000 2,826,937 140,535 11,784 653.73 1292.2
  1. a Assembly uploaded to IMG/MER and used for the phylogenetic and comparative analyses
  2. b Assembly used for metagenomic binning