The microbial community structure in industrial biogas plants influences the degradation rate of straw and cellulose in batch tests

Table 4 Clone sequences of cel5 and cel48 obtained from industrial-scale biogas processes

Clone	T-RFs (bp)	Most closely related microorganism	Identity (%)	Accession number
Cel5
OTU01	56	Ruminococcus callidus	84.8	WP_021681794
OTU02^a	85	Echinicola vietnamensis	58.0	WP_015264998
OTU03^b	85	Echinicola vietnamensis	61.4	WP_015264998
OTU04^c	106	Flavobacterium sp.	66.3	WP_007808671
OTU05	136	Niastella koreensis	73.7	AEV98714
OTU06^d	211	Marinilabilia salmonicolor	66.3	WP_036163195
OTU07^e	275	Mahella australiensis	57.4	AEE96311
OTU08^f	362	Mahella australiensis	58.4	AEE96311
OTU09	375	Clostridium sp.	72.1	WP_033166154
OTU10	396	Clostridium cellulolyticum	100	WP_015924614
Cel48
OTU01	68	Clostridium stercorarium DSM 8532	57.9	AGI39871
OTU02	68	Clostridium sp. Iso6-17a	50.0	ADM52292
OTU03	177	Ruminococcus sp. CAG:254	97.5	CCZ84184
OTU04^g	205	Clostridium sp. Iso6-24	79.0	ADM52293
OTU05	238	Ruminococcus sp. HUN007	83.2	WP_049962845
OTU06	238	Streptomyces griseorubens	61.0	WP_037642616
OTU07^h	247	Acetivibrio cellulolyticus	71.2	WP_010681059
OTU08	290	Clostridium acetobutylicum	50.5	WP_010964229
OTU09ⁱ	321	Ruminiclostridium thermocellum	75.0	ACT46162
OTU10^j	328	Clostridium termitidis CT1112	73.8	EMS73539
OTU11^k	358	Clostridium straminisolvens JCM 21531	78.8	GAE90081

^aMost closely related to uncultured bacterium AGW24153 (identity: 100 %) from laboratory-scale anaerobic reactor
^bMost closely related to uncultured bacterium AGW24153 (identity: 89.1 %) from laboratory-scale anaerobic reactor
^cMost closely related to uncultured bacterium ACV50344 (identity: 73.3 %) from lignocellulose-based sulphate-reducing bioreactor
^dMost closely related to uncultured bacterium AGO64733 (identity: 75.0 %) from anaerobic digester sludge
^eMost closely related to uncultured bacterium AEV59723 (identity: 77.0 %) from laboratory biogas digester treating rice straw
^fMost closely related to uncultured bacterium AEV59723 (identity: 76.0 %) from laboratory biogas digester treating rice straw
^gMost closely related to uncultured bacterium AGO64695 (identity: 99.0 %) from anaerobic digester sludge
^hMost closely related to uncultured bacterium AGO64692 (identity: 75.2 %) from anaerobic digester sludge
ⁱMost closely related to uncultured bacterium AGO64682 (identity: 85.6 %) from anaerobic digester sludge
^jMost closely related to uncultured bacterium AGO64673 (identity: 99.1 %) from anaerobic digester sludge
^kMost closely related to uncultured bacterium AGO64695 (identity: 99.0 %) from anaerobic digester sludge

ISSN: 2731-3654