Lessons learned from the microbial ecology resulting from different inoculation strategies for biogas production from waste products of the bioethanol/sugar industry

Table 1 Feeding regime divided into start-up and steady state according to variations on HRT and OLR.

Reactors	Inoculum	C:N ratio	Phase	Period (day)	Filter cake	Bagasse	Water	HRT (days)	OLR (g_vs L⁻¹ d⁻¹)
Reactors	Inoculum	C:N ratio	Phase	Period (day)	input (g day⁻¹)	input (g day⁻¹)	input (mL day⁻¹)	HRT (days)	OLR (g_vs L⁻¹ d⁻¹)
R3.3 R3.4	MIX	24:1	Start-up	Initial (0–41)	36.5	–	50	34.7	2.0
			Start-up	Final (42–69)	45.7	–	50	31.4	2.5
			Steady	Initial (70–113)	54.8	–	50	28.6	3.0
			Steady	Final (114–137)	54.8	–	75	23.1	3.0
R3.5 R3.6	MIX	41:1	Start-up	Initial (0–41)	15.78	6.78	50	41.4	2.0
			Start-up	Final (42–69)	19.73	8.45	50	38.4	2.5
			Steady	Initial (70–113)	23.67	10.15	50	35.8	3.0
			Steady	Final (114–137)	23.67	10.15	75	27.6	3.0
R3.7 R3.8	FCM	41:1	Start-up	Initial I (0–3)	15.78	6.78	50	41.4	2.0
				Initial II (4–20)	7.89	3.39	25	82.7	1.0
				Initial III (21–41)	15.78	6.78	50	41.4	2.0
				Final (42–69)	19.73	8.45	50	38.4	2.5
			Steady	Initial (70–113)	23.67	10.15	50	35.8	3.0
			Steady	final (114–137)	23.67	10.15	75	27.6	3.0

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