Engineering yeast with bifunctional minicellulosome and cellodextrin pathway for co-utilization of cellulose-mixed sugars

Table 1 Comparison of this work with other reports using cellulosome-engineered S. cerevisiae for cellulosic ethanol production

Pre-induction (galactose)	Added sugar for fermentation	Initial OD (A_{600 nm}) for fermentation	cEtOH Concn (g/L)	Sp. PcEtOH (mg ethanol/g cell h)	References
20 g/L	10 g/L PASC	50	1.80	~1.67	[9]
20 g/L	10 g/L CMC	50	1.00	~0.68	[10]
20 g/L	10 g/L PASC	50	1.09	~0.74	[10]
20 g/L	10 g/L PASC	50	1.90	~1.72	[11]
20 g/L	10 g/L PASC	50	2.70	~1.83	[12]
No need	20 g/L galactose + 10 g/L CMC	0.1	3.26	~62.61	This work
No need	20 g/L galactose + 10 g/L PASC	0.1	1.09	~56.37	This work

Sp. PcEtOH denotes specific productivity of cellulosic ethanol (cEtOH). Pre-induction was carried out at 20 ^oC for ~2 days. Cellulose-utilization systems: trifunctional minicellulosome without cellodextrin pathway [9–11], pentafunctional minicellulosome without cellodextrin pathway [12], bifunctional minicellulosome with cellodextrin pathway (this work)

ISSN: 2731-3654