Fig. 5
Solid-state fermentation (SSF) of A. nidulans on SEB. a Representative image of SSF. Autoclaved and dried SEB was mixed with 5 ml of liquid media without any carbon source plus 1 × 107 conidia and then incubated at 37 °C for 10 days. Proteins were extracted from solid SEB cultures. The resulting supernatants were used for the respective Megazyme assays. b Accumulative production of cellulolytic (blue) and xylanolytic (red) enzymes by the wild-type A. nidulans strain throughout the 10 days SSF. c Recovery of lignocellulolytic enzymes (predominantly xylanases) from the A. nidulans biofilm was increased in the absence of RodA or DewC, or the in the presence of the non-functional StuA1 mutation, during the SSF of SEB. Enzyme activity is presented in relation to the biomass of the fungal colony. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001