Fig. 2

Southern blot analyses of plastid transformants to select independently generated homoplasmic lines per each construct. Schematic representation of the targeting region in the plastid genome (Nt-ptDNA) and maps of transformed (Nt-DC series) plastid genome regions involved in transgene integration (a). Lines with the same number but different letters indicate transplastomic lines generated from the same primary shoot. For DC11 and DC21 transplastomic plants, heteroplasmic lines (marked with an asterisk) containing both wild-type and transformed plastomes are also shown (b). Below the maps are indicated the expected sizes of the BamHI DNA fragments in Southern blot analyses. rps12/7 and trnV correspond to the plastome integration site of the expression cassettes; aadA, aminoglycoside 3′ adenylyltransferase marker gene