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Table 2 Biotranformations of industrially pretreated A. donax biomass in the presence of commercial and plastid-based cellulose degrading enzymes

From: High-level expression of thermostable cellulolytic enzymes in tobacco transplastomic plants and their use in hydrolysis of an industrially pretreated Arundo donax L. biomass

Composition

Glucose yield (%)

Xylose yield (%)

24 h

48 h

72 h

24 h

48 h

72 h

50 °C

 Mix 1

36.84 ± 1.10

44.94 ± 0.10

51.98 ± 0.60

77.51 ± 2.90

80.66 ± 0.80

98.86 ± 3.60

 Mix 2

27.20 ± 1.30

30.46 ± 0.90

41.15 ± 1.70

72.22 ± 2.10

86.76 ± 3.70

95.84 ± 4.90

 Mix 3

19.07 ± 1.36

27.39 ± 1.62

34.21 ± 0.97

60.65 ± 2.40

74.89 ± 2.73

90.99 ± 2.91

 Mix 4

17.99 ± 0.10

26.99 ± 0.90

32.99 ± 0.10

52.97 ± 1.90

71.72 ± 0.80

92.74 ± 1.00

60 °C

 Mix 1

14.99 ± 0.46

18.93 ± 1.32

20.00 ± 0.06

45.67 ± 0.16

55.25 ± 2.36

60.53 ± 0.37

 Mix 2

18.20 ± 1.17

21.43 ± 1.40

23.05 ± 0.13

56.09 ± 3.81

66.70 ± 0.98

72.61 ± 1.22

 Mix 3

15.72 ± 0.60

18.59 ± 0.91

22.32 ± 1.13

43.26 ± 1.82

53.16 ± 2.43

63.17 ± 2.14

 Mix 4

15.23 ± 0.67

18.8 ± 0.96

20.12 ± 0.77

53.27 ± 2.09

61.83 ± 2.09

70.15 ± 1.21

  1. Mix 1: commercial mix composed by cellulase from Trichoderma reesei ATCC 26921 (5.4 U/g of pretreated biomass); β-glucosidase from Aspergillus niger (145 U/g); xylanase from Trichoderma viride (80 U/g); β-xylosidase (8 U/g)
  2. Mix 2: Mix 1, in which plastid-based xylanase (xyn) from Alicyclobacillus acidocaldarius, (80 U/g) replaced commercial xylanase from T. viride
  3. Mix 3: Mix 1, in which plastid-based β-glucosidase (celB) from Pyrococcus furiosus (145 U/g) replaced β-glucosidase from A. niger
  4. Mix 4: Mix 1, in which plastid-based xylanase (xyn) from A. acidocaldarius, (80 U/g) and β-glucosidase (celB) from P. furiosus (145 U/g) replaced xylanase from T. viride and β-glucosidase from A. niger, respectively
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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