Fig. 2

Comparative degradation of Avicel by wild-type or mutant Cel48S, either in the free state or complexed to the monovalent scaffoldin Scaf·T. Enzymatic activity is defined as mM total reducing sugars following the 48-h reaction period at 60 °C. Each reaction was performed in triplicate, and standard deviations are indicated. The red asterisk indicates the enhanced thermostabilized mutated form of the C. thermocellum Cel48S catalytic module and the pink asterisks indicate the inherently thermostable C. thermocellum cohesin and CBM