Fig. 7

Conservation of N-glycosylation sites in AN7230 and homologous proteins. Four AN7230 homologous sequences were selected to represent the slight differences into cellobiose dehydrogenase enzymes. The sequence alignment shows variation of N-glycosylated sequon position at primary sequence level in some homologous sequences. However, the structures show that the N-glycans are attached in the same loop at 3D-level (arrows). Selected proteins are from a A. nidulans AN7230 b A. kawachii (Aspka1_0182079) c A. glaucus (Aspgl1_0179713) d A. aculeatus (Aacu16872_054830) and e A. zonatus (Aspzo1_0090575). The 3D protein structures were modeled using the SWISS-MODEL [69] based on Phanerochaete chrysosporium cellobiose dehydrogenase (PDB:1KDG)