A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations

Table 2 Engineered S. cerevisiae strains constructed in this study by replacement, in the reference strain CEN.PK113-7D, of the native alleles of ASG1, ADH3, GIS4 and/or SKS1 by mutated alleles identified in mutagenized and/or evolved acetic acid tolerant strains

Strain name	Collection name	Origin of mutation	Genotype
{ASG1}^MUT1A	IMI317	MUT1A	MATa, asg1^G1248A
{ASG1,ADH3}^MUT1A	IMI318	MUT1A	MATa, asg1^G1248A, adh3^G416T
{ASG1}^MUT2B	IMI320	MUT2B	MATa, asg1^G1248T
{ASG1,ADH3}^MUT2B	IMI319	MUT2B	MATa, asg1^G1248T, adh3^T966G
{ASG1,ADH3,SKS1}^MUT2B	IMI327	MUT2B	MATa, asg1^G1248T, adh3^T966G, sks1^G821T
{GIS4}^MUT3E	IMI307	MUT3E	MATa, gis4^G1322C
{ASG1}^HAT1E	IMI316	HAT1E	MATa, asg1^A1979G
{ASG1}^HAT2A	IMI321	HAT2A	MATa, asg1^G2881C
{ASG1,ADH3}^HAT2A	IMI326	HAT2A	MATa, asg1^G2881C, adh3^T201A
{ASG1,ADH3,SKS1}^HAT2A	IMI328	HAT2A	MATa, asg1^G2881C, adh3^T201A, sks1^C617A
{GIS4}^UV-E3	IMI308	UV-E3	MATa, gis4^G295A

ISSN: 2731-3654