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Table 1 Fungal micromorphology, protein concentration and cellulase activity obtained in cultivations on lactose and wheat straw by T. reesei strains Δcre1 and QM9414

From: The micromorphology of Trichoderma reesei analyzed in cultivations on lactose and solid lignocellulosic substrate, and its relationship with cellulase production

  Cell length (µm) Cell width (µm) Branches/cell (-) FPU/mL Protein (g/L)
T. reesei Δcre1
 Wheat straw
  Agar-190 10.21 ± 3.43 3.29 ± 1.34 0.40 1.74 0.59
  Agar-150 10.08 ± 3.17 2.80 ± 0.80 0.41 0.69 0.33
  Spore-190 8.82 ± 4.18 3.85 ± 0.89 0.38 1.88 0.57
  Spore-150 9.75 ± 2.92 3.47 ± 1.06 0.48 1.82 0.30
  Agar-190-Tw 11.73 ± 3.81 3.60 ± 1.51 0.40 1.27 0.38
  Spore-190-Tw 7.87 ± 2.21 3.98 ± 1.09 0.08 2.31 0.59
  Average value 9.74 ± 1.32 3.50 ± 0.42 0.36 ± 0.14 n.c. n.c.
 Lactose
  Agar-190 11.75 ± 4.12 3.01 ± 0.77 0.43 n.d. 0.27
  Agar-150 11.67 ± 4.64 3.20 ± 0.56 0.25 n.d. 0.26
  Spore-190 10.51 ± 3.20 3.42 ± 0.97 0.28 n.d. 0.30
  Spore-150 8.55 ± 3.32 2.91 ± 1.01 0.50 n.d. 0.26
  Average value 10.62 ± 1.49 3.13 ± 0.23 0.36 ± 0.12 n.c. n.c.
T. reesei QM9414
 Wheat straw
  Agar-190 11.84 ± 3.43 3.05 ± 0.94 0.05 0.88 0.64
  Agar-150 10.21 ± 2.92 3.04 ± 1.03 0.40 0.84 0.31
  Spore-190 10.42 ± 3.15 3.32 ± 1.23 0.01 1.45 0.58
  Spore-150 10.20 ± 3.21 2.35 ± 0.95 0.26 1.64 0.26
  Agar-190-Tw 12.47 ± 3.83 2.67 ± 1.27 0.06 0.77 0.43
  Spore-190-Tw 10.09 ± 2.67 3.30 ± 1.41 0.40 1.14 0.44
  Average value 10.87 ± 1.02 2.95 ± 0.38 0.16 ± 0.15 n.c. n.c.
 Lactose
  Agar-190 16.74 ± 5.40 2.59 ± 0.69 0.25 n.d. 0.09
  Agar-150 14.24 ± 4.11 3.03 ± 1.20 0.31 n.d. 0.25
  Spore-190 13.53 ± 4.82 2.82 ± 0.64 0.23 n.d. 0.09
  Spore-150 10.59 ± 3.47 3.32 ± 0.53 0.49 n.d. 0.26
  Average value 13.77 ± 2.19 2.94 ± 0.31 0.32 ± 0.09 n.c. n.c.
  1. Volumetric cellulase activity (FPU/mL) and protein concentration represent mean values of two experiments. Dimensions of the cells represent mean values and the spread from 40 cells analyzed from one representative CLSM image. The branches per cell were analyzed in the same image and represent the value of 60–100 cells
  2. n.c. not calculated, n.d. not detectable