Fig. 1

Construction of vectors for the expression of A. cellulolyticus xylanases in C. bescii. a Schematic diagrams of Acel_0180 and Acel_0372 proteins: SP, signal peptide; GH10, a family 10 glycoside hydrolase; CBM3, a family 3 carbohydrate-binding module; CBM2, a family 2 carbohydrate-binding module. Maps of expression vectors for Acel_0180 (b) and Acel_0372 (c) expression. Genes were expressed under the transcriptional control of the C. bescii S-layer promoter. The expression vectors contain a CelA signal sequence, a C-terminal 6X his tag, a rho-independent terminator, the pyrF (from C. thermocellum) cassette for selection, and pBAS2 sequences for replication in C. bescii