Fig. 3

Adsorption isotherms of purified native SWO1 on lignocellulose components. Experiments were done at 25 °C in 50 mM sodium acetate, pH 5.0, over 2 h with shaking (500 rpm). Substrate concentration was 1 mg/mL in a total reaction volume of 200 µL. Symbols show the measured data and error bars show the SD from three independent experiments. Insets present a zoomed view on the initial data points for SWO1 adsorption on xylan and kraft lignin. The fitted Langmuir isotherms are shown as dashed lines and the corresponding parameters, maximum binding capacity related to the unit mass of substrate (B _max) and the dissociation constant K _d, are summarized in Table 2