Functional characterization of the native swollenin from Trichoderma reesei: study of its possible role as C1 factor of enzymatic lignocellulose conversion

Table 2 Summarized adsorption parameters of SWO1 on Avicel PH-101, birchwood xylan and lignin

Substrate	Avicel PH-101	Lignin	Xylan
B _max (µmol/g)	2.11 ± 0.39	25.1 ± 1.48	22.3 ± 3.13
K _d (µM)	0.89 ± 0.30	0.53 ± 0.11	0.08 ± 0.04
Absolute specificity (L/g)^a	2.4	47.4	279
Relative specifity^b	1.00	20.0	118

SWO1 showed the highest affinity and specificity for xylan followed by lignin and pure cellulose. B _max maximum binding capacity, K _d dissociation constant
^a B _max/K _d
^bAbsolute specificities normalized on Avicel PH-101

ISSN: 2731-3654