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Table 2 Comparison of pyridine nucleotide and ATP pools of E. coli BW25113 and the ΔptsGglpK* mutant

From: Elucidation of the co-metabolism of glycerol and glucose in Escherichia coli by genetic engineering, transcription profiling, and 13C metabolic flux analysis

Strain Dilution rate (h−1) NADPH (nmol/mg) NADP+ (nmol/mg) NADPH/NADP+ NADH (nmol/mg) NAD+ (nmol/mg) NADH/NAD+ ATP (nmol/mg)
Wild type 0.1 0.59 ± 0.03 0.45 ± 0.02 1.33 ± 0.09 0.43 ± 0.02 1.33 ± 0.06 0.32 ± 0.02 1193 ± 130
ptsGglpK* 0.1 0.59 ± 0.02 0.45 ± 0.01 1.33 ± 0.07 0.43 ± 0.02 1.38 ± 0.07 0.31 ± 0.02 953 ± 131
Wild type 0.35 0.77 ± 0.04 0.67 ± 0.03 1.16 ± 0.08 1.39 ± 0.06 2.76 ± 0.13 0.50 ± 0.03 11,784 ± 583
ptsGglpK* 0.35 0.35 ± 0.03 0.43 ± 0.02 0.80 ± 0.06 1.52 ± 0.07 2.17 ± 0.10 0.70 ± 0.05 2378 ± 213