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Table 2 Comparison of pyridine nucleotide and ATP pools of E. coli BW25113 and the ΔptsGglpK* mutant

From: Elucidation of the co-metabolism of glycerol and glucose in Escherichia coli by genetic engineering, transcription profiling, and 13C metabolic flux analysis

Strain

Dilution rate (h−1)

NADPH (nmol/mg)

NADP+ (nmol/mg)

NADPH/NADP+

NADH (nmol/mg)

NAD+ (nmol/mg)

NADH/NAD+

ATP (nmol/mg)

Wild type

0.1

0.59 ± 0.03

0.45 ± 0.02

1.33 ± 0.09

0.43 ± 0.02

1.33 ± 0.06

0.32 ± 0.02

1193 ± 130

∆ptsGglpK*

0.1

0.59 ± 0.02

0.45 ± 0.01

1.33 ± 0.07

0.43 ± 0.02

1.38 ± 0.07

0.31 ± 0.02

953 ± 131

Wild type

0.35

0.77 ± 0.04

0.67 ± 0.03

1.16 ± 0.08

1.39 ± 0.06

2.76 ± 0.13

0.50 ± 0.03

11,784 ± 583

∆ptsGglpK*

0.35

0.35 ± 0.03

0.43 ± 0.02

0.80 ± 0.06

1.52 ± 0.07

2.17 ± 0.10

0.70 ± 0.05

2378 ± 213

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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