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Table 1 Kinetic parameters of wild-type cADO and A121F towards C6–9 aldehydes

From: Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length

Aldehyde K m (mM) k cat (min−1) k cat /K m (mM−1min−1)
C6
 WT 0.93 ± 0.1 1.8 ± 0.1 1.9 ± 0.1
 A121F 0.96 ± 0.05 6.9 ± 0.2 7.2 ± 0.3
C7
 WT 0.59 ± 0.07 0.79 ± 0.03 1.3 ± 0.1
 A121F 0.56 ± 0.06 1.5 ± 0.1 2.7 ± 0.2
C8
 WT 0.18 ± 0.03 1.4 ± 0.06 7.8 ± 0.3
 A121F 0.18 ± 0.036 0.98 ± 0.06 5.4 ± 0.3
C9
 WT 0.069 ± 0.0087 0.61 ± 0.02 9.0 ± 0.5
 A121F 0.14 ± 0.022 0.77 ± 0.04 5.4 ± 0.3