Skip to main content

Table 1 Kinetic parameters of wild-type cADO and A121F towards C6–9 aldehydes

From: Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length

Aldehyde

K m (mM)

k cat (min−1)

k cat /K m (mM−1min−1)

C6

 WT

0.93 ± 0.1

1.8 ± 0.1

1.9 ± 0.1

 A121F

0.96 ± 0.05

6.9 ± 0.2

7.2 ± 0.3

C7

 WT

0.59 ± 0.07

0.79 ± 0.03

1.3 ± 0.1

 A121F

0.56 ± 0.06

1.5 ± 0.1

2.7 ± 0.2

C8

 WT

0.18 ± 0.03

1.4 ± 0.06

7.8 ± 0.3

 A121F

0.18 ± 0.036

0.98 ± 0.06

5.4 ± 0.3

C9

 WT

0.069 ± 0.0087

0.61 ± 0.02

9.0 ± 0.5

 A121F

0.14 ± 0.022

0.77 ± 0.04

5.4 ± 0.3

Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us