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Table 5 Detailed information on the plasmids and strains used

From: Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length

Plasmids or strains Relevant characteristics Reference
pET-28a Kanr; pBR322 ori; lacI; T7 promoter Novagen
pAL134 Kanr; pET-28b derivative containing acr1 gene; T7 promoter [34]
pKC11 Apr; pFN476 derivative containing E. coli fadD; BAD promoter [34]
pLB1593-acr1 Kanr; pET-28a derivative containing acr1 and wild-type cADO gene; T7 promoter This study
pLB1593-V184F-acr1 Kanr; pET-28a derivative containing acr1 and cADO mutant V184F gene; T7 promoter This study
BL21 (ΔfadE) BL21 (DE3) knocking out fadE [33]
LB99 BL21 (ΔfadE) bearing pAL134 and pKC11 This study
LB100 BL21 (ΔfadE) bearing pAL134, pKC11 and pLB1593-acrI This study
LB101 BL21 (ΔfadE) bearing pAL134, pKC11 and pLB1593-V184F-acrI This study