Table 5 Detailed information on the plasmids and strains used
Plasmids or strains | Relevant characteristics | Reference |
|---|---|---|
pET-28a | Kanr; pBR322 ori; lacI; T7 promoter | Novagen |
pAL134 | Kanr; pET-28b derivative containing acr1 gene; T7 promoter | [34] |
pKC11 | Apr; pFN476 derivative containing E. coli fadD; BAD promoter | [34] |
pLB1593-acr1 | Kanr; pET-28a derivative containing acr1 and wild-type cADO gene; T7 promoter | This study |
pLB1593-V184F-acr1 | Kanr; pET-28a derivative containing acr1 and cADO mutant V184F gene; T7 promoter | This study |
BL21 (ΔfadE) | BL21 (DE3) knocking out fadE | [33] |
LB99 | BL21 (ΔfadE) bearing pAL134 and pKC11 | This study |
LB100 | BL21 (ΔfadE) bearing pAL134, pKC11 and pLB1593-acrI | This study |
LB101 | BL21 (ΔfadE) bearing pAL134, pKC11 and pLB1593-V184F-acrI | This study |