Fig. 2

Enzyme activities of Man5A- and/or Mnd2A-displaying and -secreting yeast cells. a Relative β-mannanase activities of yeast transformants were measured using azo-carob galactomannan. Orange Man5A-displaying yeast cells. Green Man5A-secreting yeast cells. Gray Man5A-non-expressing yeast cells. b β-Mannosidase activities of yeast transformants were measured using p-nitrophenyl-β-d-mannopyranoside. One unit of β-mannosidase activity was defined as the amount of enzyme that liberated 1 μmol of p-nitrophenol per min under the above conditions. Orange the Mnd2A-displaying yeast cells. Green the Mnd2A-secreting yeast cells. Gray the Mnd2A-non-expressing yeast cells. Relative β-mannanase or β-mannosidase activities were calculated by normalizing the ABS₅₉₀ values or the units, respectively, per OD₆₀₀ unit