Fig. 8
Ethanol fermentations from 100 g/L 1,4-β-d-mannan and ivory nut mannan using Man5A- and Mnd2A-displaying yeast cells. a Fermentations were performed in YPA media (pH 5.0) containing 100 g/L 1,4-β-d-mannan as a carbon source. Data are presented as the mean ± standard deviation of separate cultivations (n = 3 each). b Fermentations were performed in YPA media (pH 5.0) containing 100 g/L NaOH-treated ivory nut mannan as a carbon source. Fermentations were started with an OD600 = 20 of yeast cells. Multi-copy (Man5A-Disp/Mnd2A-Disp): YPH499 harboring pFGK426-AaMan5A and pFGK424-AaMnd2A (multi-copy 2μ plasmids). Integration (Man5A-Disp-G/Mnd2A-Disp-G): YPH499 integrating pFGK406-AaMan5A and pFGK402-AaMnd2A (genomic integration plasmids)