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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance

Fig. 2

Elevated oxygen tolerance of the fusion enzyme in vitro. Survival percentage of 40 nM of purified HydA or Fd-HydA, upon 10 min of exposure to 2 different oxygen concentrations: 1.7 or 3.4 µmol. The hydrogenase activity after exposure to O2 was divided by the activity of the matching unexposed enzyme. Mean rates measured—unexposed Fd-hydA: 63.8 µmol(H2) mg(hydA)−1 min−1, Fd-HydA+ 1.7 µmol(O2): 16 µmol(H2) mg(hydA)−1 min−1, Fd-HydA+ 3.4 µmol(O2): 9.7 µmol(H2) mg(hydA)−1 min−1. Unexposed HydA: 66 µmol(H2) mg(hydA)−1 min−1, HydA+ 1.7 µmol(O2): 4.9 µmol(H2) mg(hydA)−1 min−1, HydA+ 3.4 µmol(O2): 1.2 µmol(H2) mg(hydA)−1 min−1

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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