Fig. 3
Elevated oxygen tolerance of the fusion enzyme in vivo. a O2 (blue) and H2 (orange) continuous measurements for wt strain D66, as measured by MIMS. The left window depicts the last 10 min of a dark anaerobic incubation (out of 60 min overall), whereas the right window refers to the first 100 s of illumination immediately following the incubation. b The same analysis as in (a) for the Fd-HydA expressing clone P6. c The same analysis as in (a), with a 500-µL injection of aerobic TAP-HEPES 7 min prior to illumination. d The same analysis as in c for the Fd-HydA expressing clone P6. e Survival percentage is calculated by dividing the hydrogen production rates obtained after oxygen injection (seen in panels c, d) by the matching H2 production rates obtained in anaerobiosis (seen in panels a, b). The whole experiment was repeated 3 times (biological repeats). Panels (a–d) each depict the results of a single experiment, whereas the data shown in e and the calculated p value (p < 0.05) refer to the mean over all 3 repeats