Fig. 3

Purification of Xyl2. Coomassie brilliant blue (CBB)-stained 15 % SDS-PAGE gel electrophoresis of the purification of Xyl2 by nickel affinity chromatography. The gel was stained with coomassie brilliant blue R-250. M molecular size marker. Lane 1 crude supernatant applied to the affinity resin, Lane 2 flow-through, Lane 3 wash, Lane 4 elution of Xyl2