Activity | Endo-β-(1,4)-xylanase | β-d-xylosidase |
---|
Substrate | RBB-xylan |
pNP-β-Xyl |
---|
SA (µmol min−1 mg−1)a
| Opt. pH |
K
M (mM) | Vmax (µM min−1) |
k
cat (min−1) |
k
cat/K
M (mM−1 min−1) | SA (µmol min−1 mg−1)b
| Opt. pH |
k
cat/K
M (mM−1 min−1)c
|
---|
Xyl2 | 27.2 | 7.0 − 8.0 | 40 ± 13 | 2.9 ± 0.6 | 16.0 ± 1.6 | (400 ± 2) × 10−3
| 0.19 | 6.0 | (61 ± 3) × 10−3
|
-
aSA, specific xylanase activity, calculated as μmol Remazol Brilliant Blue R liberated per mg of enzyme per minute at pH 8.0 and 40 °C
-
b
SA specific β-d-xylosidase activity, calculated as μmol pNP liberated per mg of enzyme per minute at pH 6.0 and 40 °C
-
cThe k
cat/K
M ratio for the β-d-xylosidase activity was calculated directly from a progress curve