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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Exploring complex cellular phenotypes and model-guided strain design with a novel genome-scale metabolic model of Clostridium thermocellum DSM 1313 implementing an adjustable cellulosome

Fig. 2

a Comparison of experimental and simulated metabolic fluxes for optimal growth of C. thermocellum on cellobiose without tuned growth-associated maintenance (GAM) ATP requirement. FBA simulation used only experimentally determined cellobiose uptake as a constraint. b Identification of best-fit GAM ATP requirement. The model energy balance was tuned by altering GAM ATP requirement and optimizing growth rate with specified fermentation constraints. Dotted lines frame the experimentally observed growth rate range, while solid lines illustrate the average observed growth rate and the best-fit GAM ATP requirement. c Comparison of experimental and simulated metabolic fluxes for optimal growth of C. thermocellum on cellobiose with tuned GAM ATP requirement

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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