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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Exploring complex cellular phenotypes and model-guided strain design with a novel genome-scale metabolic model of Clostridium thermocellum DSM 1313 implementing an adjustable cellulosome

Fig. 3

a Comparison of experimental and simulated metabolic fluxes for optimal growth of C. thermocellum on cellulose without ATP requirement for cellulosome synthesis. FBA simulation used experimentally measured fermentation product fluxes and the calculated GAM ATP requirement as constraints. b Identification of best-fit ATP requirement for cellulosome biosynthesis. The cellulosome ATP requirement was varied and growth rate was optimized with specified fermentation constraints. Dotted lines frame the experimentally observed growth rate range, while solid lines illustrate the average observed growth rate and the best-fit cellulosome ATP requirement. c Comparison of experimental and simulated metabolic fluxes for optimal growth of C. thermocellum on cellulose. FBA simulation used all experimental flux values as well as the best fit for GAM and cellulosome synthesis ATP requirements as constraints

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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