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Table 4 Lignin composition analysis of Norway spruce wood after 6 weeks of colonization by P. radiata

From: Time-scale dynamics of proteome and transcriptome of the white-rot fungus Phlebia radiata: growth on spruce wood and decay effect on lignocellulose

RT Peak name Spruce wood (%) After 6 weeks of fungal growth (%) Change (%)
Mean Mean
7.126 Phenol (hydroxybenzene) 0.29 ± 0.08 0.50 ± 0.09 +72
8.401 2-methylphenol 0.11 ± 0.03 0.16 ± 0.03 +45
8.753 4-methylphenol, (4-methylguaiacol) 0.25 ± 0.06 0.33 ± 0.05 +32
9.01 2-methoxyphenol, (Guaiacol) 3.33 ± 0.24 4.69 ± 0.30 +41
10.437 2-methoxy-3-methylphenol 0.76 ± 1.22 1.24 ± 0.99 +63
10.64 2-methoxy-4-methylphenol 3.19 ± 0.47 2.44 ± 0.47 −24
11.89 2-methoxy-4-ethylphenol, (ethylguaiacol) 0.83 ± 0.10 0.73 ± 0.08 −18
12.428 4-vinylguaiacol 3.17 ± 0.29 3.22 ± 0.39 +2
12.994 Eugenol, 4-allylguaiacol 0.57 ± 0.05 0.45 ± 0.02 −21
13.129 2-methoxy-4-propylphenol, (4-propylguaiacol) 0.31 ± 0.03 0.26 ± 0.04 −16
13.626 Vanillin 1.27 ± 0.56 1.45 ± 0.71 +14
13.692 2-methoxy-4-(1-propenyl)phenol, (isoeugenol, cis) 0.27 ± 0.02 0.23 ± 0.02 −15
14.274 2-methoxy-4-(1-propenyl)phenol, (isoeugenol, trans) 1.78 ± 0.18 1.47 ± 0.25 −17
14.355 1-(4-hydroxy-3-methoxyphenyl)ethanone, (guaiacylacetone) 1.06 ± 0.65 0.86 ± 0.65 −19
14.733 1-(4-hydroxy-3-methoxyphenyl)ethanone (acetovanillone) 0.98 ± 0.46 0.54 ± 0.47 −45
15.201 Phenol, 2-methoxy-4-propan-1-ol, (dihydroconiferyl alcohol) 0.81 ± 0.54 0.35 ± 0.27 −57
15.749 Coniferyl alcohol, cis 0.47 ± 0.25 0.24 ± 0.17 −49
15.867 1-(4-hydroxy-3-methoxyphenyl)propanone, (propiovanillone) 0.58 ± 0.27 0.35 ± 0.30 −40
16.657 Coniferyl alcohol, trans 0.60 ± 0.46 0.35 ± 0.46 −42
17.673 Coniferyl aldehyde 0.51 ± 0.35 0.52 ± 0.59 +2
Pyrolysis lignin (area aromatics/total) 21.3 ± 0.7 20.4 ± 2.0 −4
Gravimetric (Klason) lignin 25.8 ± 0.33 26.1 ± 0.51 +1
Acid soluble lignin 0.30 ± 0.04 0.68 ± 0.23 +127
Pch-C1,2/Pch-C3 ratioa 0.03 0.08 +167
  1. Relative peak areas (%) of lignin-derived pyrolysis product compounds identified with pyrolysis–GC/MS. Gravimetric and acid soluble lignin contents are also shown. The values are calculated from two biological replicates where 3–4 technical replicates were taken for pyrolysis samples, and from three biological replicates each of which with two technical replicate samples for Klason lignin determination
  2. aRatio of phenylmethane and phenylethane to phenylpropane type compounds