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Table 4 Primers used in this study

From: The synthetic xylulose-1 phosphate pathway increases production of glycolic acid from xylose-rich sugar mixtures

Primer

Sequence 5′→ 3′

Gene deletions by homologous recombination

 glcDEFGB_fw

GCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCCTGGACTTGAGATCGTGTAGGCTGGAGCTGCTTC

 glcDEFGB_rev

CGCGTAAACGCCAGGCGTGTAATAACGGTTCGGTATAGCCGTTTGGCTGTTTCACGCCGAGGAAGATTAAATCGCTGGCCATATGAATATCCTCCTTAG

 edd eda_fw

CGCGCGAGACTCGCTCTGCTTATCTCGCCCGGATAGAACAAGCGAAAACTTCGACCGTTCATCGTTCGCAGTTGGCATGCGGGTGTAGGCTGGAGCTGCTTC

 edd eda_rev

GCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCCATATGAATATCCTCCTTAG

 iclR_fw

CGCACCCATTCCCGCGAAACGCGGCAGAAAACCCGCCGTTGCCACCGCACCAGCGACTGGACAGGTTCAGTCTTTAACGCGTGTAGGCTGGAGCTGCTTCG

 iclR_rev

GCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGCATATGAATATCCTCCTTAG

Construction of synthetic galP promoter

 galP-KAN-fw

CCGCCCGCA CAATAACATCATTCTTCCTG ATCACGTTTCACCGCAGATTAGTGTAGGCTGGAGCTGCTTC

 galP-KAN-rev

GATAGGGACGACGTGGTGTTAGCTGTGCATATGAATATCCTCCTTAG

 galP-prom-fw

CACAGCTAACACCACGTCGT

 galP-prom-rev

ACGTCATTGCCTTGTTTGACCGCCCCTGTTTTTTAGCGTCAGGCATATAATACCTCCTAAAGTTAAACAAAATTATTTGTAG

 Prom_galPproD_veri_fw

GCTGGCCTTTTTCTTTTGGATAG

 Prom_galPproD_veri_rev

ACCGATATCCAGGCCAAAGAG

Verification Primers for gene knockouts

 xylB_fw_2

GTTATCGGTAGCGATACCGGGCATTTT

 xylB_rev_2

GGATCCTGAATTATCCCCCACCCGGTCAGGCA

 aceB_verif_fw

CATGAATCCAACGCTGGATT

 aceB_verif_rev

CGAGGCTGTTGATGTAGCC

 fw glDEF

TCCCGGACCTCGTGCACAGGTA

 glcB_verif_rev

CACACGCAGACGCAGAGTA

 gcl_verif_fw

TGTAGGTCTGAATTGCATAG

 gcl_verif_rev

CACGGGCATAACGAATCGCT

 eda_verif_rev

CCTTCCTCGGACTTCCGGTT

 eda_verif_rev

CCTTCCTCGGACTTCCGGTT

 iclR_verif2_fw

TTTCACCGCAAATACCGCCG

 iclR_verif2_rev

TGCAGCAATGTGTCGGCATAC

 arcA_verif_fw

CCTGAGGGAAAGTACCCACG

 arcA_verif_rev

GTTGTTGGGAACCAGTGTGC

 icd-verif-fw

CGACCTGCTGCATAAACACC

 icd-verif-rev

TGAACGCTAAGGTGATTGCA

Cloning of ghrA and aceA

 pACT ycdW-Fw

GAGCTCGGTACCCGGGGATCCAGGAGGCACACGATGGATATCATCTTTTATCACC

 Operon ycdW-Rev

TCATACGGTTCCTCCTTTAGTAGCCGCGTGCGCGGTCGACTT

 Operon aceA-Fw 

GCACGCGGCTACTAAAGGAGGAACCGTATGAAAACCCGTACACAACAAATTG

 pACT aceA-Rev

CTCATCCGCCAAAACAGAAGCTTTTAGAACTGCGATTCTTCAGTGGA

  1. Restriction sites are italicized and the start codons are shown in bold–italics
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