Fig. 3
CltA and CltB confer growth of S. cerevisiae in the presence of low and high concentrations of cellobiose. a CltA and CltB localize to the plasma membrane in S. cerevisiae. Strains were grown for 16 h at 30 °C in YNB supplemented with 2 % glucose before pictures were taken without (DIC) and with (GFP) fluorescence. b S. cerevisiae strains containing A. nidulans cltA, cltB, and N. crassa cdt-1 with and without the β-glucosidase-encoding (gh1-1) gene were pre-grown for 24 h in YNB media containing 1 % glucose before a serial dilution was made (1:10 dilution starting at optical density OD600nm 1.0) and diluted cells were grown on YNB plates containing different concentrations of cellobiose (0.1–2 %). c Growth curves of S. cerevisiae strains containing the N. crassa cdt-1 and the A. nidulans cltA transporter-encoding genes. Both strains also contained the β-glucosidase-encoding gene gh1-1. Strains were grown for 144 h in the presence of different concentrations of cellobiose (0.1, 1, and 2 %) at 30 °C. Growth was assessed by measuring the OD at 600 nm. The yeast strain expressing CltB has not grown in liquid medium, and it was not used in this experiment