Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris

Table 1 Mutation frequency

Template (pg)	Mn²⁺ (mM)	Total bases^a		Averaged mutation frequency (kb⁻¹)
Template (pg)	Mn²⁺ (mM)	Minimum	Maximum	Averaged mutation frequency (kb⁻¹)
50	0	452,854	697,971	0.85 ± 0.56
	1	768,351	1,250,127	1.55 ± 1.40
	2	394,019	782,970	1.98 ± 1.76
100	0	459,038	721,539	0.86 ± 0.55
	1	112,234	169,952	2.15 ± 2.08
	2	414,024	782,275	2.60 ± 4.63
250	0	141,208	223,967	1.32 ± 1.38
	1	272,842	419,288	1.98 ± 1.92
	2	220,309	433,775	2.03 ± 1.84
500	0	186,120	294,496	2.07 ± 1.95
	1	213,880	463,802	1.99 ± 1.76
	2	184,115	406,056	2.11 ± 1.92

^aThe total number of bases used for calculation of mutation frequency at each reference base of cel6A (1320 bp)

ISSN: 2731-3654