Skip to main content

Table 1 Mutation frequency

From: Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris

Template (pg) Mn2+ (mM) Total basesa Averaged mutation frequency (kb−1)
Minimum Maximum
50 0 452,854 697,971 0.85 ± 0.56
1 768,351 1,250,127 1.55 ± 1.40
2 394,019 782,970 1.98 ± 1.76
100 0 459,038 721,539 0.86 ± 0.55
1 112,234 169,952 2.15 ± 2.08
2 414,024 782,275 2.60 ± 4.63
250 0 141,208 223,967 1.32 ± 1.38
1 272,842 419,288 1.98 ± 1.92
2 220,309 433,775 2.03 ± 1.84
500 0 186,120 294,496 2.07 ± 1.95
1 213,880 463,802 1.99 ± 1.76
2 184,115 406,056 2.11 ± 1.92
  1. aThe total number of bases used for calculation of mutation frequency at each reference base of cel6A (1320 bp)