Fig. 6
Free fatty acid production and protein expression levels at various concentrations of IPTG. a Free fatty acid production of strains MG1655 expressing pBbB6c-‘tesA FT (SBF12) or pBbB6c-‘tesA R64CFT (SBF13) induced with IPTG (0–3.125 mM). Filled circles and filled diamonds indicate the FFA production of the SBF12 and SBF13, respectively. Error bars mean standard deviations of three independent experiments. b Expression levels of the ‘TesAFT and ‘TesAR64CFT measured by western blotting. Lane 1 without IPTG, lane 2 0.005, lane 3 0.025, lane 4 0.125, lane 5 0.625, lane 6 3.125 mM IPTG. The intensity values of each protein band on a western blot were determined using the ImageJ software (NIH, USA). The relative protein expression levels were calculated by dividing the intensity of each protein by the value obtained from cells induced with 0.125 mM IPTG