Recalcitrant carbohydrates after enzymatic hydrolysis of pretreated lignocellulosic biomass

Table 4 Glycosyl linkage content

Residue linkage type	Dosage (mg/g)	Glycosyl linkage content (%)
		PCS			PSCS
		0	10	100	0	10	100
Glucopyranosyl	Terminally linked (1)	5.4	3.5	6.2	7.2	9.4	7.1
	3 linked (2)	1.9	0.4	0.5	1.2	0.5	0.6
	4 linked (3)	63.3	72.1	62.8	71.6	67.8	52.7
	6 linked (4)	0.0	0.3	0.4	0.0	0.5	0.9
	4,6 linked (5)	1.6	1.8	1.7	1.5	1.8	2.3
Xylopyranosyl	4 linked (6)	13.6	11.3	13.4	8.0	8.7	13.4
	2,4 linked (7)	0.0	0.4	0.3	0.0	0.3	0.0
	3,4 linked (8)	0.9	1.3	2.0	0	1.3	3.6
Arabino(pyra/fyra)nosyl (Arap/Araf)^a	Terminally linked (9)	4.8	1.2	1.8	2.8	1.6	2.9
	Terminally linked (10)	0.9	2.0	1.7	0.4	1.7	5.3
	4 linked Arap or 5 linked Araf (11)^a	1.0	0.0	1.9	0.0	1.9	5.6
Mannopyranosyl	Terminally linked (12)	0.7	0.1	1.1	0.7	0.6	1.4
	2 linked (13)	0.0	0.0	0.7	0.0	0.0	0.9
	4 linked (14)	2.7	1.1	0.5	3.4	1.0	0.0
	6 linked (15)	0.0	0.0	0.1	0.0	0.0	0.0
Galactopyranosyl	Terminally linked (16)	0.9	1.0	0.6	0.9	0.3	0.0
	2,4 linked (17)	0.6	0.8	1.7	0.6	0.8	0.5
	3,4 linked (18)	1.4	1.7	1.6	1.4	1.1	1.7

^a 4-linked Arap and 5-linked Araf give rise to the same PMAA and can thus not be distinguished by this method. Numbers within parentheses are related with the drawings in Fig. 2
Glycosyl linkage residue of each sample at initial time (dosage 0 mg/g), and the insoluble part after enzymatic hydrolysis at lower dosage (10 mg/g) and higher dosage (100 mg/g) loading of C1 enzyme related to total dry biomass. Samples were performed in duplicate

ISSN: 2731-3654